OVERVIEW
EXPERIMENT CONSULTING
RNA ISOLATION
RNA AMPLIFICATION
PICOGRAM SOLUTIONS
MICROARRAY PROCESSING
DATA ANALYSIS
QUALITY CONTROL

GenUs BioSystems standard gene expression service includes all materials, processing, quality control, and reporting - including follow-up analysis for a single price. Specialists in small, difficult sample handling we regularly handle sample sizes down to 10 cells (Total RNA yield ~ 100 picograms) utilizing state of the art single and double amplification protocols.

Mouse Whole Genome Array - Agilent Platform

  • 41,000+ mouse genes and transcripts represented, all with public domain annotations
  • Enriched content sourced from RefSeq, RIKEN, NIA, Ensembl, UCSC Goldenpath, and Unigene databases
  • Over 70% of the represented probes are validated by Agilent's laboratory validation process
  • Sequence orientation, accuracy, and clustering assembly classification verified using NCBI genome Build 32
  • 4 X 44K slide formats printed using Agilent's 60-mer SurePrint technology
  • Total RNA Requirements Utilizing GenUs BioSystems:
    • Standard Service: > 300 nanograms Total RNA yield
    • Special Single Amp Service: > ~20 nanograms Total RNA yield
    • Special Double Amp Service > ~100 picograms Total RNA yield

Mouse Development Array - Agilent Platform
- From the lab of Dr. Minoru Ko, National Institute On Aging (NIA)

The Mouse Development Oligo Microarray 3.0 is the third generation of a whole-genome microarray designed by the laboratory of Dr. Minoru Ko at the National Institute on Aging (NIA). This microarray contains 41,999 (60-mer) probes representing all empirical and predicted mouse genes and transcripts based on NIA Mouse Gene Index. The annotation is based on NCBI Mouse RefSeq and GeneBank mRNA searches. A set of synthetic spike-in control probes are included to control variations in target labeling and hybridization, and to provide more quantitative information on transcripts.

  • 41,000+ mouse genes and transcripts represented
  • Content sourced from the NIA Mouse Gene Index
  • 4 X 44K slide formats printed using Agilent's 60-mer SurePrint technology
  • Total RNA Requirements Utilizing GenUs BioSystems::
    • Standard Service: > 300 nanograms Total RNA yield
    • Special Single Amp Service: > ~20 nanograms Total RNA yield
    • Special Double Amp Service > ~100 picograms Total RNA yield

GenUs regularly delivers dynamic range showing greater than 100,000 fold change and >5 logs. This is significanlty higher than the average 1,000 fold change and 3 logs of data provided by most labs and platforms.

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